Protocol
Recommended volume of diluted whole blood, plasma or serum:  100~400 μL / tube
  1. Optional: equilibrate PCRopsis Blood substrate with desired buffer by incubating the substrate with buffer for 5~10 minutes and removing the supernatant. However, the PCRopsis Blood substrates are ready to use and are provided pre-washed with sterile, distilled water.
  2. Tap PCRopsis Blood to ensure the substrate is at the bottom of the tube.
  3. Add 200~400 μL of sample to a PCRopsis Blood tube and gently pipette up / down to ensure complete mixing.
    1. ​Whole blood should be diluted 1:1 with PBS buffer (or other buffers) before applying to PCRopsis Blood tube.
    2. TIP: stir sample with pipette tip to ensure all of the substrate is coated by the sample
    3. TIP: lower volumes of sample result in the lowest levels of PCR inhibitors
  4. Incubate PCRopsis Blood tube with sample for 5~10 minutes at room temperature
  5. Remove cleaned lysate and use in desired PCR application
    1. TIP: insert pipette tip along the edge of the PCRopsis Blood tube until the very bottom when removing cleaned lysate
    2. TIP: wiggle pipette tip if you notice clogging 
    3. TIP: 1~7 μL of cleaned supernatant should be used per 30 μL PCR mixture

PCRopsis Blood is an alternative for:
  1. Qiagen's QIAamp DNA mini Kit
  2. Qiagen's QIAamp Circulating Nucleic Acid Kit

​Performance
The efficient PCRopsis Blood procedure enables consistent detection of DNA from various blood samples. Each lots is carefully tested to ensure maximum quality.

​Principal
PCRopsis Blood is engineered to quickly and simultaneously bind a variety of PCR inhibitors found in blood samples. These substrates do not bind to DNA. This approach allows direct detection of DNA from blood, serum or plasma without performing time-consuming DNA isolation protocols, centrifugations or other sample manipulations which may introduce mistakes, contaminants or skew the representation of DNA fragments.

​Applications
Detecting circulating DNA
​Life science research
Veterinary pathogen research
Routine applied testing

Studies with PCRopsis Blood

​(Click on the icons below for more details)

One-Step Clean-Up with ​PCRopsis Blood
PCRopsis Blood &
Magnetic bead DNA Isolation
PCRopsis Blood &
DNA Titration
PCRopsis Blood vs. 
​Qiagen's QIAamp® Blood DNA Kit
PCRopsis Blood vs.
Bio-Rad's Inhibitor-Tolerant Supermix
PCRopsis Blood &
Detection of Sepsis
PCRopsis Blood combined with 
PCRopsis Concentrator