​Pre-incubation of human plasma containing 10, 100 or 1000 cfu / sample of E. coli or S. aureus with the PCRopsis Blood for just 10 minutes allows for bacterial detection through direct PCR and amplification of a 16S rRNA fragment. No amplification was observed when the same plasma samples were directly placed into the PCR mixture without pre-incubation with PCRopsis Blood. Furthermore, this result demonstrates that PCRopsis Blood do not significantly bind to bacteria.

Note: PCR Study used PCRopsis 5x PCR Master Mix (cat. no. PMM001)