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Blood PCRopsis
​vs. Bio-Rad

The Blood PCRopsis substrate helps amplify DNA targets in plasma, even when a PCR inhibitor tolerant polymerase is used; such as, Bio-Rad's SsoAdvancedTM Universal Inhibitor-Tolerant Supermix.
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Real-time PCR studies demonstrate that Bio-Rad's inhibitor tolerant polymerase is not able to overcome PCR inhibition from 1 μL of plasma used in this study. However, pre-incubating the plasma sample with the PCRopsis Blood removed PCR inhibitors found in the plasma sample, resulting in amplification of the GAPDH target.

Note: PCR Study used PCRopsis 5x PCR Master Mix (cat. no. PMM001)
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