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One Step​ Clean-Up

Incubating diluted whole blood with the PCRopsis substrate efficiently removes PCR inhibitors and allows for the amplification of DNA. 
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​GAPDH is able to be amplified by PCR in the presence of higher volumes of whole blood when the samples is "pre-cleaned" through incubation of the sample with our PCRopsis substrate. PBS + genomic DNA was used as a control.

​Note: PCR Study used PCRopsis 5x PCR Master Mix (cat. no. PMM001)
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​Real-time PCR results show that GAPDH is only amplified from whole blood or plasma spiked with genomic DNA when the sample is pre-incubated with the PCRopsis Blood. Real-time PCR utilized 1 μL of whole blood or plasma pre-incubated with or without the PCRopsis Blood. Real-time PCR used a GAPDH specific TaqMan probe for detection of the GAPDH amplified fragment.

Note: qPCR Study used PCRopsis 5x PCR Master Mix (cat. no. PMM001)
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