3/30/2021 0 Comments
PCRopsis™ Reagent RVD + PCRopsis™ RVD Enhancer offers a direct PCR solution for specimens in most non-inactivating transport mediums.
PCRopsis™ Reagent RVD alone has been repeatedly shown to facilitate extraction-free amplification of viral, bacterial, and mammalian specimens in universal viral transport (UVT) mediums, like BD™ Universal Viral Transport system. This is one of the most common specimen transport mediums in the U.S., although many other transport mediums are used in the U.S. and abroad.
Entopsis developed PCRopsis™ RVD Enhancer to expand the compatibility of PCRopsis™ Reagent RVD to process specimens in diverse transport mediums. PCRopsis™ Reagent RVD, when used in combination with PCRopsis™ RVD Enhancer, can process specimens in just about any non-inactivating transport medium. Compatible transport mediums now include viral transport mediums recommended by the Centers for Disease Control and World Health Organization, and even a simple phosphate buffered saline (PBS) solution.
“Reagent RVD + RVD Enhancer offers a competitive price and workflow advantage to any laboratory performing PCR-based testing”, said Pratik Sharma, VP of business development at Entopsis.
2/22/2021 0 Comments
PCRopsis™ Reagent RVD-E allows for direct PCR amplification of test specimens from swabs without the need for transport mediums or lengthy RNA / DNA extraction protocols.
The first step in infectious disease testing involves swabbing a part of the body. This specimen-containing swab is placed in a tube with transport medium for shipping to a reference laboratory. The lab then performs ~10 steps to extract RNA or DNA from the transport medium for PCR testing.
The problem with this approach is that it requires many consumables that present testing bottlenecks at various points; if any are not available, then testing cannot be performed. Moreover, the multi-step process is laborious and unnecessarily error-prone; every consumable and step presents an opportunity for misdiagnosis.
What if we could skip the need for transport medium and RNA / DNA extraction?
PCRopsis™ Reagent RVD-E simplifies the testing process while saving time and lowering costs. In this new scenario, specimen-containing swabs are shipped to a testing lab in an empty transport tube. The lab then simply adds a small amount of PCRopsis™ Reagent RVD-E, vortexes the mixture and heats it. The heated sample is directly applied into downstream PCR applications.
“PCRopsis™ Reagent RVD-E will impact testing paradigms beyond COVID-19, and increase the efficiency of various other tests, including respiratory panels, wounds and drug resistance”, said Francis Lim, Ph.D., senior scientist at Entopsis.
1/2/2021 0 Comments
Reagent RVD has proven capable of amplifying SARS-CoV-2 gene targets directly from universal viral transport (UVT) medium without the need for viral RNA extraction. However, diagnostic reference laboratories also want to use this approach for the amplification of bacterial gene targets.
The PCRopsis™ research team has extended their validation efforts in this direction. It’s been confirmed that Reagent RVD is capable of mediating extraction-free qPCR of specimens containing gram positive and negative bacteria.
To date, Reagent RVD facilitates extraction-free amplification of viral, bacterial and human gene targets directly from UVT.
This opens the door to new applications with Reagent