Infectious diseases (COVID-19, flu, bacteria) can be detected by direct PCR
PCR Inhibitor Basics: why should I care?
RNA isolation is the process by which RNA is separated from viruses, cells, or sample matrices. RNA (ribonucleic acid) is a polymeric molecule essential in various biological roles in coding, decoding, regulation and expression of genes. RNA is often extracted for the purpose of downstream PCR applications.
You can learn more about RNA isolation HERE
You can learn more about RNA isolation HERE
Direct PCR / extraction-free PCR and the improved, Next Generation Direct PCR, allows for downstream PCR applications without the need for viral RNA extraction / viral RNA purification. Furthermore, direct PCR does not alter the RNA profile of your sample since it does not use 'capture and release' technology or separate components of your sample with organic solvents like Trizol, as used by solid-phase and organic RNA isolation.
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