Direct PCR is also called: PCR direct, extraction-free amplification, or direct amplification
Direct PCR: what is it and how does it help my studies?
Direct PCR is a means of DNA amplification directly from a test sample without performing RNA or DNA extraction and purification steps. As such, it is also referred to as extraction-free PCR. This approach reduces experimental time, and cost, particularly for high-volume clinical testing. It also provides a better option when faced with the challenges of amplifying a very small quantity of sample where the purification step could potentially lead to sample loss.
Extraction-free PCR works like conventional PCR. Both techniques depend on template DNA, primers and a master mix , and both use a thermal cycler for amplification. The key difference between traditional PCR and direct PCR is the need for isolation of nucleic acids. PCRopsis™ reagents allow for extraction-free PCR by utilizing novel nanotechnology to perform the following tasks in a microenvironment that's compatible with PCR:
- Lyse viruses and cells
- Disrupt nucleic acid tertiary and quaternary structures
- Disassociate proteins bound to nucleic acids
- Stabilize nucleic acids
- Sequester PCR inhibitors
Are all Direct PCR products the same?
Alternative extraction-free PCR technologies are not able to perform all of the tasks listed above in a simple and low cost fashion. This deficit in their technological approach translates to poor performance, as observed in this report. This poor performance has been a hinderance in wide-spread adoption of direct PCR techniques.
Extraction-free PCR vs Traditional RNA Extraction
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