Key Points:
- PCRopsis™ Reagent Buccal is an alternative to RNA / DNA extraction for the amplification of nucleic acids
- Offers comparable RT-qPCR / PCR results to extraction procedures for many applications
- Compatible with cheek cells dried onto buccal swab (transported without medium)
- Cost a fraction of traditional extraction procedures, with fewer steps
- Compatible with most leading RT-qPCR / PCR kits and master mixes
- Compatible with automated sample processing equipment
- Reagent Buccal is stored and shipped at room temperature
|
|
Intended Use (IN VITRO DIAGNOSTIC USE)
PCRopsis™ Reagent Buccal is intended for extraction-free PCR amplification of RNA and DNA from human buccal cells on synthetic swabs without the need for transport medium.
- Reagent Buccal may replace extraction procedures for many applications (e.g., Next generation sequencing (NGS), genotyping, life science research, veterinary pathogen research, etc.), but it must be verified by the end user.
PCRopsis™ Reagent Buccal is an alternative for:
- Thermo Fisher Scientific: various MagMAX kits
- Qiagen: QIAamp DSP DNA Kit, DNeasy Blood & Tissue Kit, etc.
- Numerous other silica- or bead-based RNA and DNA extraction kits
The Science Behind Reagent Buccal
PCRopsis™ Reagent Buccal is engineered to simultaneously bind a variety of RT-qPCR / PCR inhibitors found in buccal specimens, lyse mammalian cells, and stabilize RNA and DNA in a manner that's compatible with RT-qPCR / PCR. The product consist of a proprietary mixture of peptides, salts, stabilizers, buffers and sodium azide to achieve these tasks. Reagent Buccal allows for extraction-free amplification of RNA / DNA without performing nucleic acid isolation, centrifugations or other sample manipulations which may introduce errors, contaminants and/or skew the representation of RNA fragments.
User should verify the applicability of Reagent Buccal for unintended applications.
User should verify the applicability of Reagent Buccal for unintended applications.
Traditional RNA Extraction Results in Significantly Different Levels of miRNA ... this bias can affect results.
Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equal
Rikki A. M. Brown, Michael R. Epis, Jessica L. Horsham, Tasnuva D. Kabir, Kirsty L. Richardson & Peter J. Leedman BMC Biotechnology volume 18, Article number: 16 (2018) - Link to manuscript
A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma
Ryan K.Y. Wong, Meabh MacMahon, Jayne V. Woodside & David A. Simpson BMC Genomics volume 20, Article number: 446 (2019) - Link to manuscript
RNA Preservation Agents and Nucleic Acid Extraction Method Bias Perceived Bacterial Community Composition
Ana McCarthy, Edna Chiang, Marian L. Schmidt, Vincent J. Denef, PLOS ONE (2015) - Link to manuscript
Rikki A. M. Brown, Michael R. Epis, Jessica L. Horsham, Tasnuva D. Kabir, Kirsty L. Richardson & Peter J. Leedman BMC Biotechnology volume 18, Article number: 16 (2018) - Link to manuscript
A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma
Ryan K.Y. Wong, Meabh MacMahon, Jayne V. Woodside & David A. Simpson BMC Genomics volume 20, Article number: 446 (2019) - Link to manuscript
RNA Preservation Agents and Nucleic Acid Extraction Method Bias Perceived Bacterial Community Composition
Ana McCarthy, Edna Chiang, Marian L. Schmidt, Vincent J. Denef, PLOS ONE (2015) - Link to manuscript
Reagent Buccal does not use "capture & release" RNA / DNA technology (e.g., columns, beads, etc.), therefore it does not exhibit this bias.
Studies with PCRopsis™ Reagent Buccal
Reagent Buccal mediates direct PCR amplification of GAPDH, even when a buccal sample is diluted 1 / 10 million
|
A buccal swab was used to rub the inner cheek area of a person and then placed in a 1.5 mL tube. PCRopsis™ Reagent Buccal (200 µL) was added to the tube and vortexed 4-times, 30 seconds each time, to elute cells. The resulting eluted sample was diluted 1/10 in PCRopsis™ Reagent Buccal and each dilution heated at 95°C for 10 minutes. The processed samples (5 µL) were added to a PCR master mix (15 µL) containing primers to hGAPDH.
|
Satisfaction Guarantee - Warranty Summary
Entopsis guarantees the performance of Reagent Buccal to facilitate the direct PCR amplification of human cheek cells from dried swab specimens that have been properly collected, transported, and stored. Within 7 days of purchase, contact Entopsis (info@entopsis.com) should Reagent Buccal fail to perform as described above or in the Certificate of Analysis (CoA). In such a scenario, Entopsis will re-test the Lot in question using remaining reagent at our facility at our expense (purchaser does not need to ship product to Entopsis). If the re-tested Lot fails to meet our performance criteria, Entopsis will replace the faulty reagent free of charge. If the Lot meets our performance criteria as stated on the CoA for the Lot in question, then no additional action will be taken.
Please keep in mind that purchasers may use Reagent Buccal for numerous applications, sample types, gene targets, conditions, etc. that are untested and thereby may not work as desired. The purchaser must determine the suitability of the Reagent Buccal for its particular use. However, the Entopsis scientific team can assist in the optimization of the product for your desired application. Refunds will not be provided due to a lack of product compatibility with purchaser's desired application. This is why small test volumes of Reagent Buccal are offered.
THIS WARRANTY ONLY APPLIES IF PRODUCTS ARE PURCHASED FROM AN APPROVED VENDOR (E.G., ENTOPSIS).
Complete Terms and Conditions
Please keep in mind that purchasers may use Reagent Buccal for numerous applications, sample types, gene targets, conditions, etc. that are untested and thereby may not work as desired. The purchaser must determine the suitability of the Reagent Buccal for its particular use. However, the Entopsis scientific team can assist in the optimization of the product for your desired application. Refunds will not be provided due to a lack of product compatibility with purchaser's desired application. This is why small test volumes of Reagent Buccal are offered.
THIS WARRANTY ONLY APPLIES IF PRODUCTS ARE PURCHASED FROM AN APPROVED VENDOR (E.G., ENTOPSIS).
Complete Terms and Conditions