Key Points:
- PCRopsis™ Reagent 123 is an alternative to traditional RNA / DNA extraction
- Compatible with numerous liquid sample types (e.g., dairy, saliva, urine, swab specimens in medium, waste water, etc)
- Cost a fraction of traditional extraction procedures, with fewer steps
- Compatible with most specimen transport mediums
- Compatible with most leading RT-qPCR / PCR kits and master mixes
- Compatible with automated sample processing equipment
- Reagent 123 is stored and shipped at room temperature
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Intended Use (RESEARCH USE ONLY)
PCRopsis™ Reagent 123 is intended for nucleic acid extraction-free processing of diverse sample types.
- Reagent 123 may replace extraction procedures for many applications (e.g., life science research, veterinary, pathogen research, etc.), but it must be verified by the end user.
Certificate of Analysis:
PCRopsis™ Reagent 123 is an alternative for:
- Qiagen: QIAamp Viral RNA mini Kit
- Qiagen: QIAmp DSP Viral RNA Mini Kit
- Qiagen EZ1 Advanced XL: EZ1 DSP Virus Kit
- Qiagen EZ1 Advanced XL: EZ1 Virus Mini Kit v2.0
- QIAGEN QIAcube: QIAmp DSP Viral RNA Mini Kit
- QIAGEN QIAcube: QIAamp Viral RNA Mini Kit
- Roche MagNA Pure 24: MagNA Pure 24 Total NA Isolation Kit
- Roche MagNA Pure 96: DNA and Viral NA Small Volume Kit
- Roche MagNA Pure LC: Total Nucleic Acid Kit
- Roche MagNA Pure Compact: Nucleic Acid Isolation Kit I
- bioMérieux NucliSENS® easyMAG®
- bioMérieux EMAG®
- Thermo Fisher / Applied Biosystems: MagMAX mirVana Total RNA Isolation Kit
The Science Behind Reagent 123
PCRopsis™ Reagent 123 is engineered to simultaneously bind a variety of RT-qPCR / PCR inhibitors found in clinical specimens, lyse microorganisms and stabilize RNA and DNA in a manner that's compatible with RT-qPCR / PCR. The product consist of a proprietary mixture of peptides, salts, stabilizers, buffers, sodium azide, and proprietary nanoparticles to achieve these tasks. Reagent 123 allows for extraction-free amplification of microbial RNA / DNA without performing nucleic acid isolation, centrifugations, or other sample manipulations which may introduce errors, contaminants and/or skew the representation of RNA fragments.
User should verify the applicability of Reagent 123 for unintended applications.
User should verify the applicability of Reagent 123 for unintended applications.
Traditional RNA Extraction Results in Significantly Different Levels of miRNA ... this bias can affect results.
Total RNA extraction from tissues for microRNA and target gene expression analysis: not all kits are created equal
Rikki A. M. Brown, Michael R. Epis, Jessica L. Horsham, Tasnuva D. Kabir, Kirsty L. Richardson & Peter J. Leedman BMC Biotechnology volume 18, Article number: 16 (2018) - Link to manuscript
A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma
Ryan K.Y. Wong, Meabh MacMahon, Jayne V. Woodside & David A. Simpson BMC Genomics volume 20, Article number: 446 (2019) - Link to manuscript
RNA Preservation Agents and Nucleic Acid Extraction Method Bias Perceived Bacterial Community Composition
Ana McCarthy, Edna Chiang, Marian L. Schmidt, Vincent J. Denef, PLOS ONE (2015) - Link to manuscript
Rikki A. M. Brown, Michael R. Epis, Jessica L. Horsham, Tasnuva D. Kabir, Kirsty L. Richardson & Peter J. Leedman BMC Biotechnology volume 18, Article number: 16 (2018) - Link to manuscript
A comparison of RNA extraction and sequencing protocols for detection of small RNAs in plasma
Ryan K.Y. Wong, Meabh MacMahon, Jayne V. Woodside & David A. Simpson BMC Genomics volume 20, Article number: 446 (2019) - Link to manuscript
RNA Preservation Agents and Nucleic Acid Extraction Method Bias Perceived Bacterial Community Composition
Ana McCarthy, Edna Chiang, Marian L. Schmidt, Vincent J. Denef, PLOS ONE (2015) - Link to manuscript
Reagent 123 does not use "capture & release" RNA / DNA technology (e.g., columns, beads, etc.), therefore it does not exhibit this bias.
Satisfaction Guarantee - Warranty Summary
Entopsis guarantees the performance of Reagent 123 to mediate nucleic acid extraction-free processing of select viruses, bacteria, and mammalian cells using PCR / RT-qPCR from saliva, urine, and swab samples in compatible mediums when the sample is properly stored. Within 7 days of purchase, contact Entopsis (info@entopsis.com) should Reagent 123 fail to perform as described above or in the CoA. In such a scenario, Entopsis will re-test the Lot in question using remaining reagent at our facility at our expense (purchaser does not need to ship product to Entopsis). If the re-tested Lot fails to meet our performance criteria, Entopsis will replace the faulty reagent free of charge. If the Lot meets our performance criteria as stated on the Certificate of Analysis for the Lot in question, then no additional action will be taken.
Please keep in mind that purchasers may use Reagent 123 for numerous applications, sample types, gene targets, conditions, etc. that are untested and thereby may not work as desired. The purchaser must determine the suitability of Reagent 123 for their particular use. However, the Entopsis scientific team can assist in the optimization of Reagent 123 for your desired application. Refunds will not be provided due to a lack of product compatibility with purchaser's desired application. This is why small test volumes of Reagent 123 are offered.
THIS WARRANTY ONLY APPLIES IF PRODUCTS ARE PURCHASED FROM AN APPROVED VENDOR (E.G., ENTOPSIS).
Complete Terms and Conditions
Please keep in mind that purchasers may use Reagent 123 for numerous applications, sample types, gene targets, conditions, etc. that are untested and thereby may not work as desired. The purchaser must determine the suitability of Reagent 123 for their particular use. However, the Entopsis scientific team can assist in the optimization of Reagent 123 for your desired application. Refunds will not be provided due to a lack of product compatibility with purchaser's desired application. This is why small test volumes of Reagent 123 are offered.
THIS WARRANTY ONLY APPLIES IF PRODUCTS ARE PURCHASED FROM AN APPROVED VENDOR (E.G., ENTOPSIS).
Complete Terms and Conditions