Protocol
For each 50 μL PCR reaction, mix the following components at the indicated volumes:
Components 50 μL Reaction
Nuclease-free water 38 μL
5x PCR Master Mix 10 μL
Forward Primer (10 mM) 0.5 μL
Reverse Primer (10 mM) 0.5 μL
DNA Template 1 μL
Components 50 μL Reaction
Nuclease-free water 38 μL
5x PCR Master Mix 10 μL
Forward Primer (10 mM) 0.5 μL
Reverse Primer (10 mM) 0.5 μL
DNA Template 1 μL
PCRopsis 5x PCR Master Mix is an Alternative to:
- PCR Master Mixes for routine DNA amplification by other providers
Performance
The PCRopsis 5x PCR Master Mix outperforms or is comparable to leading market alternatives.
Applications
Routine amplification of DNA fragments
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