Protocol

For each 50 μL PCR reaction, mix the following components at the indicated volumes:
Components                  50 μL Reaction
Nuclease-free water            38 μL
5x PCR Master Mix                10 μL
Forward Primer (10 mM)    0.5 μL
Reverse Primer (10 mM)     0.5 μL
DNA Template                          1 μL

PCRopsis 5x PCR Master Mix is an Alternative to:

1. PCR Master Mixes for routine DNA amplification by other providers

​Performance

The PCRopsis 5x PCR Master Mix outperforms or is comparable to leading market alternatives.

Plasma Processed with
PCRopsis Blood:
​
7 μL plasma / PCR rxn
(primers amplify hGAPDH)

Cheek Cell Lysate Processed with
PCRopsis Cell:

7 μL lysate / PCR rxn
(primers amplify hGAPDH)

​Applications

Routine amplification of DNA fragments

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