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Fungal Samples:
PCRopsis Cell

vs
​Qiagen's QIAamp® DNA Kit

Lysing fungi from toe nail samples with PCRopsis Cell Lysis Buffer + heating and then processing lysates with PCRopsis Cell results in the detection of fungi that was
​undetectable with Qiagen's QIAamp
® DNA Kit.
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​Lysing fungal cells from toe nail samples with PCRopsis Cell Lysis Buffer + heating followed by processing with PCRopsis Cell for just 10 minutes allows for the detection of fungi that's undetectable when DNA is isolated using Qiagen's QIAamp® DNA Kit.  No amplification was observed when degenerate fungal primers were used with bacterial lysates, demonstrating these primers specifically amplify fungal DNA. 
​
Note: PCR study used PCRopsis 5x PCR Master Mix (cat. no. PMM001) and PCRopsis 5x Cell Lysis Buffer (cat. no. PCL001)
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