​Lysing bacterial, yeast and human cells with PCRopsis 5x Cell Lysis Buffer + heating followed by processing with PCRopsis Cell for just 10 minutes allows for the detection of all cell types. Qiagen's QIAamp® DNA Kit was not able to detect S. cerevisiae, but detected all other cell types. No amplification was observed when the lysate was directly placed into the PCR mixture without pre-incubation with PCRopsis Cell or DNA isolated using QIAamp® DNA Kit. 
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Note: PCR study used PCRopsis 5x PCR Master Mix (cat. no. PMM001) and PCRopsis 5x Cell Lysis Buffer (cat. no. PCL001)