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PCRopsis Cell
with Cell Lysis Buffer + Thermal Lysis
vs
​Qiagen's QIAamp® DNA Kit

Lysing cells with PCRopsis Cell Lysis Buffer + heating and then processing lysates with
PCRopsis Cell results in comparable or superior detection of DNA targets
​compared to Qiagen's QIAamp
® DNA Kit.
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​Lysing bacterial, yeast and human cells with PCRopsis 5x Cell Lysis Buffer + heating followed by processing with PCRopsis Cell for just 10 minutes allows for the detection of all cell types. Qiagen's QIAamp® DNA Kit was not able to detect S. cerevisiae, but detected all other cell types. No amplification was observed when the lysate was directly placed into the PCR mixture without pre-incubation with PCRopsis Cell or DNA isolated using QIAamp® DNA Kit. 
​
Note: PCR study used PCRopsis 5x PCR Master Mix (cat. no. PMM001) and PCRopsis 5x Cell Lysis Buffer (cat. no. PCL001)
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