Key Points:
- PCRopsis™ Activator potentiates PCRopsis™ Reagent RVD-RT
- Application: room temperature direct PCR
Intended Use (IN VITRO DIAGNOSTIC USE)
PCRopsis™ Activator is intended in combination with PCRopsis™ technology to mediate room temperature Next Gen Direct PCR™
Science Behind PCRopsis™ Activator
PCRopsis™ Activator is engineered to lyse viruses and cells, and stabilize nucleic acids at room temperature in a manner that’s compatible with PCRopsis™ Next Generation Direct PCR™ reagents. This buffer uses a proprietary mixture of chemicals to achieve these tasks.
Studies with PCRopsis™ Activator
PCRopsis™ Activator + Reagent RVD-RT allows for the amplification of 3.9 viral copies / µL from transport medium containing SARS-CoV-2
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Transport medium was spiked with inactivated SARS-CoV-2 (ATCC) and processed with Reagent RVD-RT (with Activator). Five viral gene sites were amplified through RT-qPCR, in duplicates, and averages shown. Reagent RVD-RT mediated amplification of tested gene regions with Ct values between 34 ~40 at 3.9 viral copies / µL.
RT-qPCR was performed using IDT qPCR probe assay and Promega GoTaq® Probe 1-Step RT-qPCR System. |
Satisfaction Guarantee - Warranty Summary
Entopsis guarantees the performance of PCRopsis™ Activator, in combination with Reagent RVD-RT, to mediate room temperature direct PCR. Within 7 days of purchase, contact Entopsis (info@entopsis.com) should PCRopsis™ Activator fail to perform as described above or in the CoA. In such a scenario, Entopsis will re-test the Lot in question using remaining product at our facility at our expense (purchaser does not need to ship product to Entopsis). If the re-tested Lot fails to meet our performance criteria, Entopsis will replace the faulty product free of charge. If the Lot meets our performance criteria as stated on the Certificate of Analysis for the Lot in question, then no additional action will be taken.
Please keep in mind that purchasers may use PCRopsis™ Activator for numerous applications, sample types, gene targets, conditions, etc. that are untested and thereby may not work as desired. The purchaser must determine the suitability of PCRopsis™ Activator for their particular use. However, the Entopsis scientific team can assist in the optimization of PCRopsis™ Activator for your desired application. Refunds will not be provided due to a lack of product compatibility with purchaser's desired application.
THIS WARRANTY ONLY APPLIES IF PRODUCTS ARE PURCHASED FROM AN APPROVED VENDOR (E.G., ENTOPSIS).
Complete Terms and Conditions
Please keep in mind that purchasers may use PCRopsis™ Activator for numerous applications, sample types, gene targets, conditions, etc. that are untested and thereby may not work as desired. The purchaser must determine the suitability of PCRopsis™ Activator for their particular use. However, the Entopsis scientific team can assist in the optimization of PCRopsis™ Activator for your desired application. Refunds will not be provided due to a lack of product compatibility with purchaser's desired application.
THIS WARRANTY ONLY APPLIES IF PRODUCTS ARE PURCHASED FROM AN APPROVED VENDOR (E.G., ENTOPSIS).
Complete Terms and Conditions